ha tag 1f5c6 Search Results


anti ha  (Proteintech)
96
Proteintech anti ha
a Frequency bar chart showing the charge of 1840 scFv interdomain linker sequences in the NCBI database. b Increased solubility of an anti-VDAC1 scFv with charged interdomain linker (upper). Sample processing controls (lower), n = 3 biological replicates, (G 4 S) 4 /HA vs (G 4 S) 4 /HA/3FLAG P = 0.001, (G 4 S) 4 /HA/3FLAG vs (G 4 D) 4 /HA/3FLAG P = 0.43 (two-tailed, two-sample equal variance t test). c Coulombic electrostatic potential colouring of negatively charged interdomain linker structural representations. d Co-immunoprecipitation of GFP-FUS/TLS protein by Z-FUS-derived scFv intrabodies with varying charge inter-domain linkers bound by <t>immobilised</t> <t>anti-HA</t> beads and visualised by SDS-PAGE/western blot. Antibodies used to probe western membranes are stated to the right. Images showing anti-GFP and anti-FUS blots were run on separate gels. e Percent solubility and abundance in the soluble fraction of scFv intrabodies shown in ( d ), n = 3 biological replicates, (G 4 S) 4 /HA vs (G 4 D) 4 /HA/3FLAG solubility P = 0.0007, abundance P = 0.0002 (two-tailed, two-sample equal variance t test). Blue – serine linker, green – aspartate linkers, purple – glutamate linkers. f , g High solubility engineered into scFv intrabodies, derived from 4E6 ( P = 0.0001, two-tailed, two-sample equal variance t test) and anti-p231-235 ( P = 0.0016, two-tailed, two-sample equal variance t test) antibodies, targeting tau with combined N- and C-terminal tags and linker modifications, 4E6 n = 4 and 5 biological replicates. p231–235 n = 3 and 5 biological replicates. Lower panels are sample processing controls. All error bars represent one standard deviation. Source data are provided as a Source Data file. I – Insoluble fraction, S – Soluble fraction.
Anti Ha, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha+tag+1f5c6/pmc12963631-208-4-7?v=Proteintech
Average 96 stars, based on 1 article reviews
anti ha - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
ha tag 1f5c6  (Proteintech)
95
Proteintech ha tag 1f5c6
a Frequency bar chart showing the charge of 1840 scFv interdomain linker sequences in the NCBI database. b Increased solubility of an anti-VDAC1 scFv with charged interdomain linker (upper). Sample processing controls (lower), n = 3 biological replicates, (G 4 S) 4 /HA vs (G 4 S) 4 /HA/3FLAG P = 0.001, (G 4 S) 4 /HA/3FLAG vs (G 4 D) 4 /HA/3FLAG P = 0.43 (two-tailed, two-sample equal variance t test). c Coulombic electrostatic potential colouring of negatively charged interdomain linker structural representations. d Co-immunoprecipitation of GFP-FUS/TLS protein by Z-FUS-derived scFv intrabodies with varying charge inter-domain linkers bound by <t>immobilised</t> <t>anti-HA</t> beads and visualised by SDS-PAGE/western blot. Antibodies used to probe western membranes are stated to the right. Images showing anti-GFP and anti-FUS blots were run on separate gels. e Percent solubility and abundance in the soluble fraction of scFv intrabodies shown in ( d ), n = 3 biological replicates, (G 4 S) 4 /HA vs (G 4 D) 4 /HA/3FLAG solubility P = 0.0007, abundance P = 0.0002 (two-tailed, two-sample equal variance t test). Blue – serine linker, green – aspartate linkers, purple – glutamate linkers. f , g High solubility engineered into scFv intrabodies, derived from 4E6 ( P = 0.0001, two-tailed, two-sample equal variance t test) and anti-p231-235 ( P = 0.0016, two-tailed, two-sample equal variance t test) antibodies, targeting tau with combined N- and C-terminal tags and linker modifications, 4E6 n = 4 and 5 biological replicates. p231–235 n = 3 and 5 biological replicates. Lower panels are sample processing controls. All error bars represent one standard deviation. Source data are provided as a Source Data file. I – Insoluble fraction, S – Soluble fraction.
Ha Tag 1f5c6, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha+tag+1f5c6/pm41642933-248-2-11?v=Proteintech
Average 95 stars, based on 1 article reviews
ha tag 1f5c6 - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier

Image Search Results


a Frequency bar chart showing the charge of 1840 scFv interdomain linker sequences in the NCBI database. b Increased solubility of an anti-VDAC1 scFv with charged interdomain linker (upper). Sample processing controls (lower), n = 3 biological replicates, (G 4 S) 4 /HA vs (G 4 S) 4 /HA/3FLAG P = 0.001, (G 4 S) 4 /HA/3FLAG vs (G 4 D) 4 /HA/3FLAG P = 0.43 (two-tailed, two-sample equal variance t test). c Coulombic electrostatic potential colouring of negatively charged interdomain linker structural representations. d Co-immunoprecipitation of GFP-FUS/TLS protein by Z-FUS-derived scFv intrabodies with varying charge inter-domain linkers bound by immobilised anti-HA beads and visualised by SDS-PAGE/western blot. Antibodies used to probe western membranes are stated to the right. Images showing anti-GFP and anti-FUS blots were run on separate gels. e Percent solubility and abundance in the soluble fraction of scFv intrabodies shown in ( d ), n = 3 biological replicates, (G 4 S) 4 /HA vs (G 4 D) 4 /HA/3FLAG solubility P = 0.0007, abundance P = 0.0002 (two-tailed, two-sample equal variance t test). Blue – serine linker, green – aspartate linkers, purple – glutamate linkers. f , g High solubility engineered into scFv intrabodies, derived from 4E6 ( P = 0.0001, two-tailed, two-sample equal variance t test) and anti-p231-235 ( P = 0.0016, two-tailed, two-sample equal variance t test) antibodies, targeting tau with combined N- and C-terminal tags and linker modifications, 4E6 n = 4 and 5 biological replicates. p231–235 n = 3 and 5 biological replicates. Lower panels are sample processing controls. All error bars represent one standard deviation. Source data are provided as a Source Data file. I – Insoluble fraction, S – Soluble fraction.

Journal: Nature Communications

Article Title: Reliable repurposing of the antibody interactome inside the cell

doi: 10.1038/s41467-026-69057-0

Figure Lengend Snippet: a Frequency bar chart showing the charge of 1840 scFv interdomain linker sequences in the NCBI database. b Increased solubility of an anti-VDAC1 scFv with charged interdomain linker (upper). Sample processing controls (lower), n = 3 biological replicates, (G 4 S) 4 /HA vs (G 4 S) 4 /HA/3FLAG P = 0.001, (G 4 S) 4 /HA/3FLAG vs (G 4 D) 4 /HA/3FLAG P = 0.43 (two-tailed, two-sample equal variance t test). c Coulombic electrostatic potential colouring of negatively charged interdomain linker structural representations. d Co-immunoprecipitation of GFP-FUS/TLS protein by Z-FUS-derived scFv intrabodies with varying charge inter-domain linkers bound by immobilised anti-HA beads and visualised by SDS-PAGE/western blot. Antibodies used to probe western membranes are stated to the right. Images showing anti-GFP and anti-FUS blots were run on separate gels. e Percent solubility and abundance in the soluble fraction of scFv intrabodies shown in ( d ), n = 3 biological replicates, (G 4 S) 4 /HA vs (G 4 D) 4 /HA/3FLAG solubility P = 0.0007, abundance P = 0.0002 (two-tailed, two-sample equal variance t test). Blue – serine linker, green – aspartate linkers, purple – glutamate linkers. f , g High solubility engineered into scFv intrabodies, derived from 4E6 ( P = 0.0001, two-tailed, two-sample equal variance t test) and anti-p231-235 ( P = 0.0016, two-tailed, two-sample equal variance t test) antibodies, targeting tau with combined N- and C-terminal tags and linker modifications, 4E6 n = 4 and 5 biological replicates. p231–235 n = 3 and 5 biological replicates. Lower panels are sample processing controls. All error bars represent one standard deviation. Source data are provided as a Source Data file. I – Insoluble fraction, S – Soluble fraction.

Article Snippet: Membranes were probed with anti-HA (clone 6I21 Proteintech 81290-1, clone 1F5C6 Proteintech 66006-2), anti-FLAG (clone 8H6A10 Proteintech HRP-66008) or anti-myc (clone 4A6 Merck 05-724), anti-FUS/TLS (clone 1B4F8 Proteintech 68262-1), anti-SOD1 (clone 2F10G1 Proteintech 67480-1), anti-α-synuclein (clone 1B10E9 Proteintech 66412-1), anti-UCHL1 (clone 1C9E11 Proteintech 66230-1) antibodies with appropriate secondary antibodies (LI-COR IRDye 680RD goat anti-rabbit 926-68071, IRDye 800CW goat anti-mouse 926-32210, Proteintech HRP-conjugated goat anti-mouse SA00001-1 or Invitrogen HRP-conjugated goat anti-rabbit A16104).

Techniques: Solubility, Two Tailed Test, Immunoprecipitation, Derivative Assay, SDS Page, Western Blot, Standard Deviation

a 3B5H10-derived scFv intrabodies created using native, human redesigned (VL K20D, VL K42D, VH K13D, VH K19D, VH K23E), and ProteinMPNN SOL inverse folded variable domains with stated tags, linkers and model noise. Fixed residue positions CDR only: VL 24–34, 50–56, 89–97 and VH 25–32, 52–56, 95−102. Extended: VL 24–36, 47–56, 86–97 and VH 26–59, 91–105. b Percent of 3B5H10-derived scFv found in the soluble cell fraction. n = 3, 6, 3, 5, 2, 5, 5, 6, 2 biological repeats. c In-cell thermal stability assay of 3B5H10-derived scFv intrabodies. Stability for construct 1 could not be measured. Representative of n = 2 biological replicates. d Abundance of 3B5H10-derived scFv in the soluble fraction. n = 3 biological replicates. e Co-immunoprecipitation of GFP-polyQ74 by 3B5H10-derived scFv intrabodies bound by immobilised anti-HA beads and visualised by SDS-PAGE/western blot. Antibodies used to probe western membranes are stated to the left. f SDS-PAGE/western blot showing the solubility and g Intracellular thermal stability of MS785-derived scFv intrabodies. h Co-immunoprecipitation of A4V SOD1 by MS785-derived scFv (AI redesign 020 ) intrabody bound by immobilised anti-HA beads and visualised by SDS-PAGE/western blot. i Quantification of A4V SOD1 immunoprecipitation by MS785 showing robust binding across pH 7.0–8.0 with a non-significant preference for physiological pH, n = 3 biological replicates, P = 0.284 (one way ANOVA with Dunnett’s post hoc analysis). Antibodies used to probe western membranes are stated to the left. Chothia numbering throughout. Error bars represent one standard deviation. Hs – human, AI – ProteinMPNN SOL inverse folding. Source data are provided as a Source Data file. Western blots are representative of at least two experiments.

Journal: Nature Communications

Article Title: Reliable repurposing of the antibody interactome inside the cell

doi: 10.1038/s41467-026-69057-0

Figure Lengend Snippet: a 3B5H10-derived scFv intrabodies created using native, human redesigned (VL K20D, VL K42D, VH K13D, VH K19D, VH K23E), and ProteinMPNN SOL inverse folded variable domains with stated tags, linkers and model noise. Fixed residue positions CDR only: VL 24–34, 50–56, 89–97 and VH 25–32, 52–56, 95−102. Extended: VL 24–36, 47–56, 86–97 and VH 26–59, 91–105. b Percent of 3B5H10-derived scFv found in the soluble cell fraction. n = 3, 6, 3, 5, 2, 5, 5, 6, 2 biological repeats. c In-cell thermal stability assay of 3B5H10-derived scFv intrabodies. Stability for construct 1 could not be measured. Representative of n = 2 biological replicates. d Abundance of 3B5H10-derived scFv in the soluble fraction. n = 3 biological replicates. e Co-immunoprecipitation of GFP-polyQ74 by 3B5H10-derived scFv intrabodies bound by immobilised anti-HA beads and visualised by SDS-PAGE/western blot. Antibodies used to probe western membranes are stated to the left. f SDS-PAGE/western blot showing the solubility and g Intracellular thermal stability of MS785-derived scFv intrabodies. h Co-immunoprecipitation of A4V SOD1 by MS785-derived scFv (AI redesign 020 ) intrabody bound by immobilised anti-HA beads and visualised by SDS-PAGE/western blot. i Quantification of A4V SOD1 immunoprecipitation by MS785 showing robust binding across pH 7.0–8.0 with a non-significant preference for physiological pH, n = 3 biological replicates, P = 0.284 (one way ANOVA with Dunnett’s post hoc analysis). Antibodies used to probe western membranes are stated to the left. Chothia numbering throughout. Error bars represent one standard deviation. Hs – human, AI – ProteinMPNN SOL inverse folding. Source data are provided as a Source Data file. Western blots are representative of at least two experiments.

Article Snippet: Membranes were probed with anti-HA (clone 6I21 Proteintech 81290-1, clone 1F5C6 Proteintech 66006-2), anti-FLAG (clone 8H6A10 Proteintech HRP-66008) or anti-myc (clone 4A6 Merck 05-724), anti-FUS/TLS (clone 1B4F8 Proteintech 68262-1), anti-SOD1 (clone 2F10G1 Proteintech 67480-1), anti-α-synuclein (clone 1B10E9 Proteintech 66412-1), anti-UCHL1 (clone 1C9E11 Proteintech 66230-1) antibodies with appropriate secondary antibodies (LI-COR IRDye 680RD goat anti-rabbit 926-68071, IRDye 800CW goat anti-mouse 926-32210, Proteintech HRP-conjugated goat anti-mouse SA00001-1 or Invitrogen HRP-conjugated goat anti-rabbit A16104).

Techniques: Derivative Assay, Residue, Stability Assay, Construct, Immunoprecipitation, SDS Page, Western Blot, Solubility, Binding Assay, Standard Deviation

a Design rules for reformatting an antibody as an optimised scFv intrabody. b Intracellular target specificity of 672 scFv constructed using the principles in ( a ). c Mean amino acid conservation for native to inverse folded scFv intrabody and charged residues in non-fixed positions within variable domain consensus sequences. d Charge frequency histograms for VL and VH framework sites before and after inverse folding. e Anti-p53 scFv 11D3 and 421 constructed using rules in ( a ) are highly soluble. Lower pane are sample processing controls. f Co-immunoprecipitation of p53 by anti-p53 scFv intrabodies. g Co-immunoprecipitation of UCHL1 by anti-UCHL1 scFv intrabodies. h Co-immunoprecipitation of GFP by anti-GFP scFv intrabody derived from Neuromabs antibody N86_38. All immunoprecipitation visualised by SDS-PAGE/western following immobilisation with anti-HA beads. Antibodies used to probe western membranes are stated to the left. IP Immunoprecipitation. Chothia numbering throughout. Western blots are representative of at least two experiments.

Journal: Nature Communications

Article Title: Reliable repurposing of the antibody interactome inside the cell

doi: 10.1038/s41467-026-69057-0

Figure Lengend Snippet: a Design rules for reformatting an antibody as an optimised scFv intrabody. b Intracellular target specificity of 672 scFv constructed using the principles in ( a ). c Mean amino acid conservation for native to inverse folded scFv intrabody and charged residues in non-fixed positions within variable domain consensus sequences. d Charge frequency histograms for VL and VH framework sites before and after inverse folding. e Anti-p53 scFv 11D3 and 421 constructed using rules in ( a ) are highly soluble. Lower pane are sample processing controls. f Co-immunoprecipitation of p53 by anti-p53 scFv intrabodies. g Co-immunoprecipitation of UCHL1 by anti-UCHL1 scFv intrabodies. h Co-immunoprecipitation of GFP by anti-GFP scFv intrabody derived from Neuromabs antibody N86_38. All immunoprecipitation visualised by SDS-PAGE/western following immobilisation with anti-HA beads. Antibodies used to probe western membranes are stated to the left. IP Immunoprecipitation. Chothia numbering throughout. Western blots are representative of at least two experiments.

Article Snippet: Membranes were probed with anti-HA (clone 6I21 Proteintech 81290-1, clone 1F5C6 Proteintech 66006-2), anti-FLAG (clone 8H6A10 Proteintech HRP-66008) or anti-myc (clone 4A6 Merck 05-724), anti-FUS/TLS (clone 1B4F8 Proteintech 68262-1), anti-SOD1 (clone 2F10G1 Proteintech 67480-1), anti-α-synuclein (clone 1B10E9 Proteintech 66412-1), anti-UCHL1 (clone 1C9E11 Proteintech 66230-1) antibodies with appropriate secondary antibodies (LI-COR IRDye 680RD goat anti-rabbit 926-68071, IRDye 800CW goat anti-mouse 926-32210, Proteintech HRP-conjugated goat anti-mouse SA00001-1 or Invitrogen HRP-conjugated goat anti-rabbit A16104).

Techniques: Construct, Immunoprecipitation, Derivative Assay, SDS Page, Western Blot

a Assessment of the interaction between scFv intrabodies derived from anti-SOD1 conformation specific antibodies with wild-type, A4V, G93A and D90A SOD1 by co-immunoprecipitation on anti-HA beads and visualised by SDS-PAGE/western blot. Antibodies used to probe western membranes are stated on the left and right. b SOD1 conformation specific intrabodies target five distinct epitopes in the SOD1 monomer here shown in the fully metalated disulphide intact form. c Immunoprecipitation of A4V SOD1 by MS785-derived scFv but not Z-FUS-derived scFv. d Oligomeric state specificity of five anti-α-synuclein antibodies. e Co-immunoprecipitation of α-synuclein by scFv intrabodies derived from antibodies in ( d ) on anti-HA beads and visualised by SDS-PAGE/western blot. Antibodies used to probe western membranes are stated. IP Immunoprecipitation.

Journal: Nature Communications

Article Title: Reliable repurposing of the antibody interactome inside the cell

doi: 10.1038/s41467-026-69057-0

Figure Lengend Snippet: a Assessment of the interaction between scFv intrabodies derived from anti-SOD1 conformation specific antibodies with wild-type, A4V, G93A and D90A SOD1 by co-immunoprecipitation on anti-HA beads and visualised by SDS-PAGE/western blot. Antibodies used to probe western membranes are stated on the left and right. b SOD1 conformation specific intrabodies target five distinct epitopes in the SOD1 monomer here shown in the fully metalated disulphide intact form. c Immunoprecipitation of A4V SOD1 by MS785-derived scFv but not Z-FUS-derived scFv. d Oligomeric state specificity of five anti-α-synuclein antibodies. e Co-immunoprecipitation of α-synuclein by scFv intrabodies derived from antibodies in ( d ) on anti-HA beads and visualised by SDS-PAGE/western blot. Antibodies used to probe western membranes are stated. IP Immunoprecipitation.

Article Snippet: Membranes were probed with anti-HA (clone 6I21 Proteintech 81290-1, clone 1F5C6 Proteintech 66006-2), anti-FLAG (clone 8H6A10 Proteintech HRP-66008) or anti-myc (clone 4A6 Merck 05-724), anti-FUS/TLS (clone 1B4F8 Proteintech 68262-1), anti-SOD1 (clone 2F10G1 Proteintech 67480-1), anti-α-synuclein (clone 1B10E9 Proteintech 66412-1), anti-UCHL1 (clone 1C9E11 Proteintech 66230-1) antibodies with appropriate secondary antibodies (LI-COR IRDye 680RD goat anti-rabbit 926-68071, IRDye 800CW goat anti-mouse 926-32210, Proteintech HRP-conjugated goat anti-mouse SA00001-1 or Invitrogen HRP-conjugated goat anti-rabbit A16104).

Techniques: Derivative Assay, Immunoprecipitation, SDS Page, Western Blot